Praveen Yadav, an accused in the high-profile (MPPEB) scam, allegedly committed suicide on Wednesday, by hanging himself at his residence in Madhya Pradesh's Morena.
Praveen, a resident of Maharajpur village was charged in 2012 in connection with the Vyapam scam.
More than 40 people associated with the scam have died since the story broke in 2013.
The deaths include accused and witnesses as well as a journalist who was investigating the story, and have largely been under mysterious circumstances.
The death includes the Dean of a Jabalpur Medical College Dr Arun Sharma and the other is Aaj Tak journalist Akshay Singh, who was covering the scam.
The scam involved 13 different exams conducted by Vyapam, for selection of medical students and state government employees (including food inspectors, transport constables, police personnel, school teachers, dairy supply officers and forest guards). The exams were taken by around 3.2 million students.
Cases of irregularities in these entrance tests had been reported since the mid-1990s, and the first FIR was filed in 2000.
However, until 2009, such cases were not thought to be part of an organised ring. When major complaints surfaced in the pre-medical test (PMT) in 2009, the state government established a committee to investigate the matter. The committee released its report in 2011, and over a hundred people were arrested by the police.
The Central Bureau of Investigation (CBI) on Thursday filed a charge sheet against 592 persons in the VYAPAM scam case.
As per the CBI sources, the charge sheet mentions the names of four ex-VYAPAM officials, including the then director Pankaj Trivedi and former system analysts, Nitin Mohindra, Ajay Kumar Sen and CK Mishra.
The VYAPAM scam is an admission and recruitment fraudulent scheme, beginning in 1995, involving politicians, senior officials and businessmen in Madhya Pradesh Professional Examination Board (MPPEB).
The accused allegedly employed imposters to write papers, supply forged answer sheets and bribed officials to manipulate exam hall sitting arrangements.
Earlier on October 31, the premier investigating agency had filed a charge sheet against 490 accused in the scam.
The chairmen of four private medical colleges have also been named in the latest charge sheet.
These include Ajay Goenka of Chirayu medical college, SN Vijaywargiya of Peoples Medical College, JN Chowkse of LN medical college and one Suresh Singh Bhadauria.